100 watt push pull.
I have my layout divided roughly into regions:

I'm worried about three separate things.
1. Region 1: This is where some power amp components come close to some first stage preamp components. For example, the first stage cathode resistor ground side is about 1/2 inch away from a screen resistor power supply side. I could scoot my power amp region a bit to the right, but I would end up with a bit of wasted pcb space. I've also been considering doing it in two boards, the preamp, and everything else.
2. Region 2: The presence and depth controls of the PI come pretty close to the reservoir caps. I'm guessing the presence and depth caps are less sensitive to noise than say, the preamp components, but I'm guessing also the reservoir caps are radiating noise like crazy due to the current pulses that charge them.
3. Long signal wires from the PI to the coupling caps of the power amp. These are really the only long signal traces on the board. Left side is about 4 inches, right side is about 3 inches. I could run these as shielded wire if necessary. I doubt they are too susceptible to noise as the signal is pretty high amplitude to drive the power tubes of course, but long traces I know aren't ideal.
I'm totally fine with just trying it and seeing how it goes, but if there are any obvious mistakes I'm making I could fix them now.